We describe the spatial distribution, population structure, and fecundity of Ceratozamia matudai in two populations in the El Triunfo Biosphere Reserve, in Chiapas, Mexico. Three random plots of 20 × 20 m were laid out in a cypress forest habitat of the cycad and six plots in a cloud forest habitat on Mount Ovando. The aggregated spatial pattern of C. matudai showed a clumped local distribution on shallow soils on steep slopes. Individuals between the sites showed differences in leaf production, trunk diameter, height, and growth pattern. A population structure with an inverse “J” curve was obtained in both sites. Emergence of male and female cones appears to be synchronous in both populations, with a predominance of male cones recorded.